Abcam
Anti-gamma H2A.X (phospho S139) antibody - ChIP Grade (ab2893)
ab2893
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We are using human dental fibroblast cells to study oxidants. We measure oxidant induced DNA damage by induction of H2AX in cell lysates.
Western Blot
Human dental fibroblast cell lysates
1:1000 in 5% milk
5% Milk for 1 hr
1:10,000 dilution anti-rabbit conjugated with HRP secondary antibody
NA
ECL based detection
We were able to identify the target protein band in the blots without any treatment and when we exposed the cells to oxidants we were able to see induction of H2AX phosphorylation.
None
Good H2AX antibody for immunoblot analysis
There are some nonspecific bands observed when the blots are probed after stripping
Works well for human cell lysates to test the levels of gH2AX phosphorylation.