Good Kit for Silver Staining

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Overall

Quality of Results

Ease-of-Optimization

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Company:

Life Technologies

Product Name:

SilverQuest Silver Staining Kit

Catalog Number:

LC6070

This is a good, easy to use kit for silver staining protein samples. I have used this kit to detect femtomol concentrations of proteins and then successfully sent them off for mass spec. analysis.

Experimental Design and Results Summary

Application

Silver Staining

Starting Material

None

Protocol Overview

Load and run protein samples on polyacrylamide gels (I use premade ones) and then follow the kits instructions for visualization. After visualization you can cut out the bands and send for mass spec.

Tips

Take precautions to not contaminate with keratin

Results Summary

The kit works very well to visualize protein samples and with clean backgrounds. Samples can also be use in mass spec. downstream after destaining.

Additional Notes

None

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Summary

The Good

Easy to use and works

The Bad

Price

The Bottom Line

This kit works well for silver staining.

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