Santa Cruz Biotechnology
Search Results Actin Antibody (I-19): sc-1616
sc-1616
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We had problems in identifying a good Actin antibody that works for both human and mouse samples. Based on our search for actin antibody, we found this Santa Cruz Biotechnology Ab works well as mentioned in the data sheet for both human and mouse samples. We use this Ab as our housekeeping control for all the western blots we perform in house.
Western Blot
Human gingival fibroblast cell lysates
1:1000 in 5% milk
5% Milk for 1 hr
1:10,000 dilution anti-goat conjugated with HRP secondary antibody
NA
ECL based detection
We found this actin antibody works well for human tissue and cell lysates
None
Very good Actin antibody for human samples
None noted
Works both for human and mouse samples