Cayman Chemicals
Cox-2 Antibody
160112
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We are using Cox-2 as a marker for increased proinflammatory response when we identify PGE2 is induced by oxidants in our cell culture medium. We found COX-2 was induced by treatment of cells with oxidants in the cell lysates.
Western Blot
Human fibroblasts cells
1:1000 in 5% milk
5% Milk for 1 hr
1:10,000 dilution anti-mouse conjugated with HRP secondary antibody
NA
ECL based detection
Very clear Cox-2 band observed at 72 kDa.
None
Reliable Cox-2 antibody for human samples
Works only for human not for mouse samples
Good cox-2 antibody for human samples - Immunoblot analysis