Vector Labs
Monoclonal anti-CD3
VP-RM01
Browse Similar Products: CD3 Antibodies
The aim of my research is to study different immune cell populations in the intestines. T-cells, of course, are found among the immune cells in the gut and are one of the most abundant immune cell populations in the gut. To visualize differences in T-cell infiltration or number in healthy vs diseased states, I wanted to perform immunofluorescence.
Immunofluorescence
Mouse intestine (pre-fixed, frozen)
1:400 in 1% BSA overnight at 4 degrees
5% goat serum in 1% BSA in PBS
Goat anti-rabbit AlexaFluor594 was used at 1:200 in PBS and was incubated on the section for 1 hour at room temperature in the dark
None
Zeiss LSM 700 confocal
The stain was very obvious with very little background. T-cells are found in the lamina propria and in-between the epithelial cells, which is the expected distribution of T-cells in the gut. Additionally, the staining looks like receptor staining, which is expected given the localization of CD3 in the T-cell.
Great staining with very little background.
Nothing to mention.
The product offers very clean staining of CD3 and is great for identifying T-cells through immunofluorescence.