Life Technologies
Hoechst 33342
H3570
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Cells treated with growth factors were immunostained for p21 to test its nuclear localization. Nucleus of cells was stained with Hoechst 33342 to check co-staining with p21.
To test nuclear localization of p21 protein.
DPBS, 4% paraformaldehyde, 0.5% triton X-100, 0.3% BSA, Anti-p21, goat anti-rabbit Alexa fluor 588, mounting medium.
Mammalian cells grown on cover slip were fixed in paraformaldehyde, permiabilized, blocked with BSA and immunostained with p-21 Ab. PSB washed cells were stained with Hoechst 33342 (dilution of 1:1000) for 5 min and mounted in slide. Slides were observed with fluroscence microscope and images were captured.
Use Hoechst33342 dilution in PBS (1:1000)
Got very good nuclear staining.
None
Easy to use and gives best staining.
Excellent dye.
Product Name: Hoechst 33342Supplier Page