Good BmtI Restriction Enzyme

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Overall

Quality of Results

Ease-of-Optimization

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Company:

New England Biolabs

Product Name:

BmtI

Catalog Number:

R0658S

This is a good source of the BmtI restriction enzyme. I use this product when doing diagnostic restriction digests or in molecular cloning when I need to cut out an insert.

Experimental Design and Results Summary

Application

Restriction Digest

Starting Material

Plasmid DNA

Protocol Overview

Add restriction enzyme to mixture of correct NEBuffer with plasmid DNA and incubate at 37 celcius for a sufficient amount of time to digest all plasmid DNA.

Tips

Use the correct NEBuffer

Results Summary

The BmtI enzyme worked well cutting the vector 1 time (expected single cutter) and excised an insert in the vector that was flanked by BmtI and XbaI sites.

Additional Notes

None

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Summary

The Good

Easy to use

The Bad

Nothing

The Bottom Line

This is a good source of BmtI restriction enzyme. Another great product from NEB.

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