Overexpression of FoxM1 in Glioblastoma, Breast Cancer and Colorectal Cancer

chemical biology
Indian institute of chemical technology
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Company:

Cell Signaling Technology

Product Name:

FoxM1 (D12D5) XP®

Catalog Number:

Rabbit mAb #5436

Info: View Product View Product Specs

Browse Similar Products: FoxM1 Antibodies

We are working on FoxM1's role in colorectal cancer and trying to find out the molecular mecanism. This product is user-friendly and allows you to detect your specific band without any non-specificity.

Experimental Design and Results Summary

Applications

Western Blot

Sample

colo205 cell lysate

Primary Incubation

1:1000 dilution, 2hr at room temperature.

Blocking Agent

Non fat milk

Secondary Incubation

1hr at room temperature,1:5000 dilution

Tertiary Incubation

None

Detection

ECl from GE,chemi -doc

Results Summary

We found in aggressive stage colorectal cancer, FoxM1 expression is more 5 folds higher than control.

Additional Notes

Run on 10 percent or 8 percent gel

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Summary

The Good

It produces a very sharp band at the correct size

The Bad

There is a small band below the FoxM1 band

The Bottom Line

It is a very good antibody and I would recommend if for anybody to use.

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