Prevent Mycoplasma Contamination

Brigham and Women's Hospital
Pathology
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma-Aldrich

Product Name:

LookOut Mycoplasma Erase

Catalog Number:

L1420-500ML

This spray is a cleaning solution that prevents and eliminates mycoplasma from lab surfaces. Most of our cell lines were contaminated with Mycoplasma, which goes un-noticed unless tested using PCR based methods. We use this product to disinfect our lab space and TC hoods. Ever since, we haven't observed myocplasma contamination in our cultures.

Experimental Design and Results Summary

Application

Disinfection

Starting Material

Any surface

Protocol Overview

Spray the entire surface area, leave it for 5-10 seconds and then wipe it with clean towel.

Tips

Use 70% ethanol to clean any precipitates that may have formed

Results Summary

Our cultures have been free from Mycoplasma for several months now.

Additional Notes

None

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Summary

The Good

Great product

The Bad

Leaves precipitates if left to dry

The Bottom Line

A recommended disinfectant for all labs working with cell cultures.

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