Sigma-Aldrich
IgG1, Kappa murine myeloma
M9269
To accurately measure the concentration of murine IgG1 in a sample, an accurate standard is required. On quantitative ELISAs, multiple IgG1 standards were tested, yielding strong reproducible standard curves using this IgG1, Kappa murine myeloma.
Quantitative ELISA
Monoclonal MOPC 21, purified immunoglobulin, buffered aqueous solution
This IgG1 standard was dissolved to a concentration of 1000 ng/ml in PBS/1%BSA/0.05%Tw20.
Confirm protein concentration by OD 280nm reading
This monoclonal yielded reproducible standard curves, between ELISA plates and between experiments, and is easy to use as it's provided at close to 1 mg/ml concentration.
None
Availability, price, efficacy
This IgG1 kappa from a murine myeloma provided accurate, reproducible IgG1 stndard curves for the quantification of IgG1 in murine samples via ELISA.