Flow Cytometric Detection of Kappa Light Chain FITC

August 31, 2015

Microbiology and Immunology
University of Miami
Post-doctorall fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BD Biosciences

Product Name:

Kappa light chain FITC

Catalog Number:

550003

Our lab is concerned with studying the transcriptome and signaling changes that occur during transitional B-cell development. This is a major checkpoint in peripheral tolerance where autoreactive B-cell clones are purged. We used Kappa light chain FITC from BD to assess the kappa / lambda light chain ratio in splenocytes. This antibody was easy to use and gave great separation.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

C57BL/6 splenocytes

Primary Incubation

15-20min in FACS buffer 1:200

Blocking Agent

Fc block 5min prior

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

Kappa light chain FITC was tested on both BM and splenocytes and provided great separation from non-light chain expressing cells.

Additional Notes

Needs optimization due to strong signal

Related Categories

Image Gallery

Summary

The Good

Kappa light chain FITC is a powerful antibody displaying a wide spectrum over the log axis

The Bad

Needs optimization in terms of concentration due to potential compensation issues

The Bottom Line

This antibody performed well and would be recommended for analyzing kappa versus lambda light chain ratios.

Share your experience with other scientists. Write a Review! »

Join the discussion