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Lambda light chain Biotin
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Our lab is concerned with studying the transcriptome and signaling changes that occur during transitional B-cell development. This is a major checkpoint in peripheral tolerance where autoreactive B-cell clones are purged. This particular study examined the kappa to lambda light chain ratio in splenocytes.
Flow Cytometry
C57BL/6 splenocytes
15-20min in FACS buffer 1:200 biotinylated antibody
Fc block 5min prior
15-20min in FACS buffer 1:300 final concentration for SAV BV711
NA
Flow cytometry
Lambda light chain biotin antibody provided great separation
None
Lambda light chain biotin works great.
Each SAV conjugate will require separate optimization
This antibody provides great separation over many logs and can be used with many SAV fluorochrome conjugates