BD Biosciences
CD86 PE
553692
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Browse Similar Products: CD86 Flow Cytometry Antibodies
Our lab is concerned with studying transcriptome and signaling changes that occur during transitional B-cell development. This is a major checkpoint in peripheral tolerance where autoreactive B-cell clones are purged. Typically, splenocytes from C57BL/6 and various mutant strains are used.
Flow Cytometry
C57BL/6 splenocytes
15-20min in FACS buffer
Fc block 5min prior
NA
Flow cytometry
C57BL/6 mice were ip injected with Listeria Monocytogenes. Splenocytes were collected 18-48 hours later and subsequently stained for flow cytometry. CD86 expression on B-cells typically becomes elevated upon activation. Results show CD86 surface expression is increased on B-cells (both immature and mature).
1:200 final dilution for 1 million cells
CD86 PE is able to detect activation of B-cells
This antibody gives reliable detection of CD86 which ranged in expression over a fairly wide spectrum