FACS Grade Anti-Mouse CD25-PE

August 26, 2015

Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

PE- conjugated Anti-mouse CD25

Catalog Number:

12-0251-81

Info: View Product View Product Specs

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I am working on autoimmunity and I primarily use this antibody for the characterization of CD4+CD25+Foxp3+ Treg cells from mouse splenocytes.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse CD25+ enriched CD4+T cells

Primary Incubation

1:500 in 100uL FACS Buffer, 4 degrees for 1h

Blocking Agent

FACS buffer containing 2% BSA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Becton Coulter CyAn

Results Summary

I got nice staining and CD4+CD25+ population with good purity.

Additional Notes

None

Related Categories

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Summary

The Good

Ease of optimization and good quality

The Bad

High cost and cannot be used in common for human and rat

The Bottom Line

Worth buying this antibody.

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