eBioscience
Anti-Hu IgG - (Fc Gamma Specific)-PE
12-4998-82
Info: View Product View Product Specs
Browse Similar Products: IgG Antibodies
In our lab we produce many proteins with Fc tag. I primarily use this antibody for the screening of (human) IgG tagged protein producing clones.
Flow Cytometry
Fc-Tagged protein expressing 293T cells
1:500 in 100uL FACS Buffer 4 degrees for 1h
NA
Flow cytometry using BD CyAn instrument
I got very good results for my FACS based screening of my clones. There was a clear distinction between Fc tag expressing Vs non expressing cells.
None
Ease of optimization and quality of results
Not much except high cost
Very good antibody for human IgG Fc staining.