Sigma-Aldrich
Red blood cell lysis buffer
R7757
This buffer is intended to remove red blood cells from cell suspensions obtained from blood or secondary lymphoid organs. The incubation times are longer than comparative products and even after two rounds of lysis, I saw significant amount of Red cells in my suspension.
Red blood cell lysis
Cell suspension
Pellet cells and resuspend in 2-3mL of red blood cell lysis buffer. Incubate at room temperature for 5 minutes followed by wash with appropriate buffer. The cells are ready for downstream applications
When working with blood, perform lysis twice with washes in between
Poor efficiency of lysis was observed and comparable products have better efficiency with shorter incubation times.
None
Easy to use
Inefficient
Test different lysis buffers to compare or make your own with a traditional recipe.