Cell Signaling Technology
Phospho-FoxO1 (Thr24)/FoxO3a (Thr32) Antibody
9464
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This antibody was used to detect phospho- Fox03a/Fox01 in response to growth factor treatment in mammalian endothelial cell lines.
Western Blot
Mammalian endothelial cell protein extract
1:1000 (5%BSA in TBST), Overnight at 4 degrees Celsius
5% Skimmed milk in TBST
1:4000 (5%skimmed milk in TBST), 2h at RT
NA
ECL
Time dependent increase of pFox03a was observed. However, bands were not clear and of low quality.
None
Good specificity, no background was detected
Got dusty bands
Overall good antibody for immunobloting.