CD21 FITC Flow Stain

August 17, 2015

Microbiology and Immunology
University of Miami
Post-doctorall fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

CD21

Catalog Number:

553818

I am studying splenic B-cell development and tolerance mechanisms. The goals of my experiment were to phenotype spleens from various genotypes. This CD21 FITC flow cytometric stain is excellent for gating on marginal zone B cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes

Primary Incubation

20min ice

Blocking Agent

Fc Block 5min

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

BD CD21 FITC flow stain worked well at 1:200.

Additional Notes

None

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Summary

The Good

CD21 FITC stain works great

The Bad

None

The Bottom Line

CD21 FITC is great for flow cytometry and can be used at 1:200 final concentration.

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