Superb Antibody to Study IDH2 Related Disease Mechanism

chemical biology
Indian institute of chemical technology
SRF

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma-Aldrich

Product Name:

IDH2 antibody

Catalog Number:

SAB1100103

Browse Similar Products: IDH2 Antibodies

We used this antibody to study IDH2 mutation which has a role in Glioblastoma such as grade 3 and grade 4 tumours.

Experimental Design and Results Summary

Applications

Western Blot

Sample

U-87 cell line

Primary Incubation

2hr at room temperature

Blocking Agent

Non-fat milk

Secondary Incubation

1 hr

Tertiary Incubation

NA

Detection

ECL from Amersham

Results Summary

We got beautiful blots which are publishable quality without any non-specificity or failure.

Additional Notes

None

Related Categories

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Summary

The Good

No non-specificity

The Bad

If you perform the primary incubation for too long, you will get non-specific bands.

The Bottom Line

Outstanding, cost-effective antibody. Reduce the work burden without non-specificity and without fail.

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