Selleck Chemicals
Romidepsin
S3020
The aim of this project was to use HDAC inhibitors in an attempt to recapitulate HIV-1 latency reversion, but in mice. Romidepsin was used to assess whether it had effects on viral vectored vaccines. This was used in vivo, using luciferase reporter and IVIS. Romidepsin had been previously used in mice, macaques and humans to activate the viral reservoir.
In vivo
Romidepsin Powder
Romidepsin was solubilised in 10% DMSO, 90% PBS prior to IP administration in BALB/c mice.
Mix thoroughly in DMSO prior to adding of PBS to avoid precipitation
Administration of romidepsin significantly increased luciferase transgene expression from viral vector, compared to vehicle alone.
None
Availability, price
Has to be solubilised
Great product for in vivo use of this HDACi