Detection of ASPN in Cancer Cells

Cancer bio
MDA
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Abcam

Product Name:

Anti-Asporin antibody

Catalog Number:

ab31303

Info: View Product View Product Specs

Browse Similar Products: ASPN Antibodies

Our longstanding goal is to understand the role of proteins in cancer progression. Here we detect the expression of ASPN protein in osteosarcoma cell lines and understand the localization of the protein. From the results it is evident that the cells do express this protein and is cytoplasmic in nature. This is a good antibody to detect the expression of ASPN in cancer cells using immunofluoroscence.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human osteosarcoma cell lines

Primary Incubation

1:500 in block buffer

Blocking Agent

0.05% FBS in PBS

Secondary Incubation

Alexa Fluor 488

Tertiary Incubation

Nucleus stain

Detection

Using Zeiss confocal microscope

Results Summary

Green color indicates the localization of ASPN.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Works well

The Bad

Polyclonal

The Bottom Line

No background observed.

Share your experience with other scientists. Write a Review! »

Join the discussion