Novus
Alcohol dehydrogenase Antibody
NBP1-45335
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Browse Similar Products: Alcohol Dehydrogenase Antibodies
This antibody was used in a study that investigated the role of acetaldehyde in alcohol induced lipodystrophy in rats. It has been published in Alcoholism: Clinical and Experimental Research 2015 (Zhang et al., Visceral white adipose tissue is susceptible to alcohol-induced lipodystrophy in rats: role of acetaldehyde, 2015, 39: 416-423). Several alcohol dehydrogenase antibodies were tested in this study for various applications; NBP1-45335 gave best results in the Western blot using liver homogenates.
Western Blot
Liver homogenates
Alcohol dehydrogenase antiboy (Novus, NBP1-45335), incubated 12 hours at 4°C in blocking reagent (1:1000)
5% non-fat milk
Horseradish peroxidase conjugated anti-rabbit IgG (Sigma, A0545), 1h incubation at room temperature (1:10,000)
NA
Chemiluminescent, (supersignal west dura extended duration substrate, Prod# 34076, Thermo), Fujifilm (LAS-4000)
This antibody worked excellently in Western blot analysis. Band is very specific.
None
Highly specific, low background
Cannot be used for FACS.
This is a very good antibody to detect ADH in the liver. The background is low. However, this antibody might not be suitable for FACS.