Abcam
Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody
ab4819
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We are performing compound toxicity and signalling studies on diverse neuronal cell lines. This product was used to check the expression of pERK in Neurotrophicy of N2A cells.
Western Blot
Neuroblastoma (N2A) cells
1:4000, Room Temperature, 2h:30min.
5%BSA in 1xTBST(0.1%TritonX100)
Antirabbit-HRP, 2h, Room temperature
NA
Chem-doc was used to detect the chemiluminiscence
pERK expression levels are high in neurotrophicy of N2A cells.
Can be used in 4C overnight (primay antibody incubation)
No non-specific bands, dilution is more (saves antibody)
High cost compared to the same product from other companies.
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