Sigma-Aldrich
ANA-12
SML0209-5MG
We are performing compound toxicity and signalling studies on diverse neuronal cell lines. We used this product to check the role of BDNF-TrkB in neurotrophic activity. The product is specific to the TrkB inhibition.
Neurotrophic activity
Neurobalstoma cells (N2A cells)
Cells were plated on coverslips in a 6-well plate (10000 cells/cm2), after 24h of plating, cells were changed with serum free medium, after 6h cells were treated with ANA-12 (10uM) for 24h, cells were fixed in 4% PFA and bright field images were captured.
Concentration of ANA-12 need to be standardised.
Compared to untreated cells, in ANA-12 treated N2A cells the Neurite outgrowth is less.
None
Easy to study the BDNF-TrkB role Neuronal cells using the product
High cost
Suggested to use for checking the role of TrkB.