BD Biosciences
B220 V500 Clone RA3-6B2
561226
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I am studying splenic B-cell development and tolerance mechanisms. The goals of my experiment were to phenotype spleens from various genotypes. To separate B-cells from other leukocytes I used the B220 V500 antibody from BD. This antibody was great because most of the other antibodies I use are not V500 conjugated. The separation is great with a dilution range of 1:200 to 1:400 per million cells. I would highly recommend this product for any surface B-cell stain. Note: this antibody is not great in intracellular stains, likely due to V500.
Flow Cytometry
Splenocytes
15-20 min
Fc block 5 min prior
NA
Flow cytometry
B-cell discrimination from other splenocytes
Dilution 1:200 to 1:400 FACS buffer (2% FBS + 2mM EDTA)
Separates B-cells very well
Does not work with intracellular stain
Great surface stain for B-cells.