Cell Signaling Technology
Phospho-Stat3 (Tyr705) Antibody
9131S
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The aim of the experiment was to test the effect of dominant negative (tyr705) STAT3 (Ad-dnSTAT3) on STAT3 (Tyr705) phosphorylation in HEK293A cells.
Western Blot
Whole cell lysate from HEK293A cells infected or not infected with Ad-dnSTAT3
1:1000 (5%BSA in TBST), Overnight at 4 degrees celsius
5% Skimmed milk in TBST
1:4000 (5% skimmed milk in TBST), 2h at RT
NA
ECL
STAT3 phosphorylation at Tyr705 was significantly reduced with overexpression of dominant negative STAT3 in HEK293A cells.
No nonspecific band or background was detected
Excellent antibody