Good Quality Real-Time PCR System

National Dairy Research Institute
Animal Biochemistry
Scientist

Overall

Performance

Ease-of-Optimization

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Company:

Life Technologies

Product Name:

7500 fast Real time PCR system

Catalog Number:

4351106

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We have a 7500 Fast Real-Time PCR System in our central lab equipment facility. The aim of my experiment was to study the quantitative expression of various genes in control, diabetic, and milk-fed rat tissues.

Experimental Design and Results Summary

Application

qPCR

Starting Material

Rat cDNA isolated from tissue

Protocol Overview

Preincubation 50°C, 20 sec 95°C, 10 sec Amplification (40 cycles) Denaturation 95°C, 15 sec Annealing and extension 60°C, 1 min Melt curve 95°C, 15 sec 60°C, 1 min 95°C, 30 sec 60°C, 1 min Cooling 4°C, Pause/Infinite

Tips

None

Results Summary

Cattle milk has a putative role in diabetes mailtus

Features Summary

None

Additional Notes

None

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Summary

The Good

Easy to operate

The Bad

None

The Bottom Line

Best machine for qPCR

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