Great Anti-Laminin Antibody

otolaryngology
Northwestern University
Post doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-Laminin antibody

Catalog Number:

ab11575

Info: View Product View Product Specs

Browse Similar Products: Laminin Antibodies

We wanted to localize/visualize the basement membrane. As laminin is a protein found in the basement membrane we used this ab to visualize it. In addition, we wanted to determine whether there was co-localization between laminin and complement products.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

OCT embedded frozen human nasal sinus tissue

Primary Incubation

1/250, overnight at 4 C

Blocking Agent

10 % Donkey serum, 0.1 % triton X 100 in PBS

Secondary Incubation

Donkey anti-rabbit 1/800, 1 h, RT

Tertiary Incubation

NA

Detection

Fluorescent microscopy

Results Summary

We could detect laminin in human sinus tissue.

Additional Notes

None

Related Categories

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Summary

The Good

Great for frozen sections

The Bad

None

The Bottom Line

Great for frozen sections

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