Good A7L6 Antibody

otolaryngology
Northwestern University
Post doc

Overall

Quality of Results

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Company:

Thermo Scientific Pierce Antibodies

Product Name:

Large Heparan Sulphate Proteoglycan Monoclonal Antibody (A7L6)

Catalog Number:

MA1-06821

Info: View Product View Product Specs

Browse Similar Products: A7L6 Antibodies

We wanted to see where in the human nasal sinuses we could detect heparan sulfate.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Frozen human nasal sinus tissue imbedded in OCT

Primary Incubation

1/50, overnight at 4 C

Blocking Agent

10 % Donkey serum , 0.1 % triton X 100 in PBS

Secondary Incubation

Donkey anti-rat 1/800

Tertiary Incubation

NA

Detection

Fluorescent microscopy

Results Summary

Heparan sulfate can be detected in the glands and in the basement membrane of nasal sinus tissue

Additional Notes

None

Related Categories

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Summary

The Good

Great for frozen sections

The Bad

None

The Bottom Line

Great antibody for immunohistochemistry of frozen sections!

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