Life Technologies
RNAiMax
13778075
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Our research explores the interaction of drug and gene delivery vehicles with intracellular trafficking processes such as those associated with endosomal and cholesterol trafficking. In particular, we were interested in transiently knocking down these trafficking proteins such as clathrin, caveolin, ARF6, flotillin amongst others. We used RNAimax as the liposomal delivery agent for the corresponding siRNA for these proteins and got excellent knockdown of these proteins
Gene Delivery Studies
HeLa cells, corresponding custom-ordered siRNA from Dharmacon
Simple incubation protocols were followed to form complexes between the siRNA and RNAiMax as detailed in the associated manual.
Dropwise addition of solutions helps
A representative image from our experiments is shown of the cell samples wherein we measured clathrin expression. The Western blot using the clathrin, caveolin, laminim (non-specific siRNA) and noRNA control siRNA clearly shows that siCLT(anti-clathrin siRNA) knocks down clathrin but the other siRNA have no effect on the clathrin expression.
None
Very specific knockdown. Easy to use and stores well.
Easy knockdown of your target mRNA via the use RNAimax
Product Name: Lipofectamine RNAiMAX Transfection ReagentSupplier Page