Dual-Luciferase Reporter Gene Assay

upmc
pathology
research associate

Overall

Performance

Ease-of-Optimization

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Company:

Berthold Technologies USA, LLC

Product Name:

The Lumat LB 9507 tube luminometer

Catalog Number:

LB 9507

I do work with liver pathology and have a strong background and experience in innovative liver tumor models, molecular biology techniques such as cloning, mutagenesis and gene expression along with cell and tissue culture. In my lab, we usually use this product to detect the gene interaction with dual-luciferase gene assay

Experimental Design and Results Summary

Application

Dual-Luciferase Reporter Gene Assay

Starting Material

Transit transfection cell lysis and dual luciferase assay kit reagents

Protocol Overview

1. Transfect the cells when cell confluence is about 60-70%. 2. Harvest the cells after 24, 36, & 48 hs after transfection. 3 Follow the luciferase kit assay protocol and assay the interaction. 4. Set up assay protocol based on the requirement.

Tips

Just follow the protocol and the assay can be finished in 2 hours when added with the reagent

Results Summary

The results are stable when the assay finished in 2 hours and we only used it for Dual-Luciferase Reporter Gene Assay. However, it also could be used for other types of experiments, so people can use it in different ways based on the experiment machine introduction online

Features Summary

None

Additional Notes

None

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Summary

The Good

Works well

The Bad

None

The Bottom Line

This machine is very good, and the assay volume could be reduced to 50ul and thus people can save some of their assay kit.

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