ELISpot feasibility

June 26, 2015

Northwestern
Neurological Surgery
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioScience

Product Name:

Human IFN gamma ELISpot ready set go

Catalog Number:

88-7386-21

Overall the product has proven to be effective even though the description lacks the identification of required reagents that must be purchased to utilize the kit. Despite a few shortcomings, customer service has proven to be very helpful with troubleshooting.

Experimental Design and Results Summary

Application

Immunomonitoring

Starting Material

Cultured human glioblastoma cells and autologous/non-autologous patient T-cells separated with a magnetic bead system (positive selection)

Protocol Overview

The protocol was very easy to follow. Additionally, they provide an easy format to go over dilutions.

Tips

Review the protocol prior to purchase to note the required additional reagents that need be purchased

Results Summary

Results are reliable but plates should be air dried at least overnight. Also, there may be a distinct orange hue in the background and the actual result may not appear as indicated on the website.

Additional Notes

None

Image Gallery

Summary

The Good

Easy to use, well written protocol, great technical support

The Bad

Missing reagents not included in the kit that were not specified on the website prior

The Bottom Line

Recommended kit. Although eBioScience should note the required reagents that are missing in the kit. Also, they should provide the user with recommended ELISpot reader parameters.

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