New England Biolabs
dam-/dcm- Competent E. coli
c2925h
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I use these competent cells when I need to produce a DNA plasmid that is free of dam or dcm methylation. This is important when I need to perform restriction digests on plasmid sequences that would have restriction digests inhibited by methylation.
Competent Cells
None
Follow manufacturer protocol to transform cells
Make sure to add enough DNA for good transformation efficiency
These competent cells produce colonies containing non-methylated plasmid DNA
Produce methylation free DNA
This is a good product
Product Name: dam-/dcm- Competent E.coliSupplier Page