Use of Isotemp 205 waterbath

University of Nebraska Medical Center
Pharmacology and Experimental Neuroscience
Graduate Research Assistant

Overall

Performance

Ease-of-Optimization

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Company:

Fisher Scientific

Product Name:

Isotemp 205

Catalog Number:

15-462-S5Q

Browse similar products: Water baths

I am conducting research on Parkinson’s disease, looking specifically at how effector T cells are able to modulate and exacerbate the neurodegeneration seen in the substantia nigra of Parkinson’s patients. This Fisher Scientific Isotemp 205 waterbath was used in the heat inactivation of FBS.

Experimental Design and Results Summary

Application

Cell culture

Starting Material

FBS

Protocol Overview

Fetal Bovine Serum is thawed from -20 degrees C to room temperature. The FBS is then heated in the waterbath at 56 degrees C for 30 minutes before being aliquoted and frozen at -20 degrees C again.

Tips

Do not leave the FBS in the heated waterbath for longer than the alloted time.

Results Summary

The FBS is completely heat inactivated after 30 minutes in the heated waterbath.

Features Summary

None

Additional Notes

None

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Summary

The Good

Waterbath heats up quickly

The Bad

None

The Bottom Line

The Fisher Scientific waterbath is a great choice when shopping for waterbaths because it heats up quickly and it maintains its heat at the designated setting consistently.

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