Miltenyi
CD4+CD25+ Regulatory T Cell Isolation Kit, mouse
130-091-041
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This Kit is very useful for isolating CD4+CD25+ Tregs from lymph nodes and spleen in order to culture Tregs and use them for RT PCR or for adoptive cell transfer experiments. I especially use it for performing in vivo Treg suppression assays, in which you transfer Tregs together with naive CD4+ T cells into immunodeficient RAG-/- mice. In this assay, the Tregs should prevent the colitogenic activity of the transferred naive CD4+ T cells.
Isolation of Tregs
Lymph nodes, spleen
Protocol is used as described in manufacturer's instructions, no changes were done
None
The purity of the isolated Tregs was around 90-95%. The total cell number per 1 mouse (using all LNs and Spleen together) of isolated cells represent 1-3 million cells
You can isolate the cells and use them for further cell culture experiments
Expensive
I prefer this product to isolate Tregs in contrast to sorting, since it is quite fast and efficient.