Cell Signaling Technology
PIAS1 XP Rabbit mAb Antibody
3550
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PIAS1, an E3-type SUMO ligase tethering SUMO moiety to substrate proteins, is crucial for protein functions and transcriptional coregulation in different cellular pathways. PIAS1-mediated SUMOylation of PML promotes its degradation through ubiquitin-proteosome pathway. PIAS1 is also involved in DNA damage response promoting the accrual of SUMO-2/3 at DNA damage sites and confers ionizing irradiation resistance. This antibody is used to detect PIAS1 in Western blotting to confirm the successful silence of PASI1 by siRNA.
Western Blot
HeLa cell line with or without PIAS1 siRNA
1:1000 at room temperature for 2hr
5% non-fat milk in TBS-Tween20
1:5000 Anti-Rabbit-HRP
NA
Bio-Rad ChemiDoc Imager
PIAS1 antibody recognizes a band around 75 kDa in control siRNA, but not in PAIS1 siRNA treated samples indicating the complete gene knockdown of PASI1.
None
Clean and specific
Very nice antibody.