Cell Signaling Technology
Rabbit anti-V5 tag (D3H8Q)
13202
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This antibody was used to perform immunofluorescence microscopy on HeLa cells transfected with a V5 containing construct. Several anti-V5 antibodies from different host species were used. D3H8Q was used in parallel with several mouse anti-V5 antibodies. D3H8Q gave minimal background fluorescence and strong immunoreactivity for epifluorescence microscopy.
Immunofluorescence
HeLa cells transfected with anti-V5 containing construct
D3H8Q (rabbit anti-V5) incubated 1 hour in PBS 1% + normal goat serum +0.1% Triton X-100 at a dilution of 1:500
PBS 1% + normal goat serum +0.1% Triton X-100
Alexa 594 conjugated goat anti-rabbit IgG (Life technologies cat. no A-11012) at a dilution of 1:500
NA
Epifluorescence microscopy, Nikon
This antibody worked well in this application. The staining was similar to several mouse anti-V5 antibodies used. The use of rabbit anti-V5 antibodies can expand multicolor applications with the use of different host secondary antibodies.
This antibody also worked in SW480 cells with similar results. Different Alexa Fluor conjugated secondaries also produced similar results.
Highly specific
The antibody is somewhat pricey
This antibody is a good choice for a non mouse anti-V5 monoclonal antibody. It is highly specific and sensitive.