AbD Serotec (a Bio-Rad company)
CD68 Antibody | FA-11
MCA1957
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Macrophages can be identified in tissue cross-sections via the CD68 intracellular marker. We used this product along with the use of Cell Signaling Tech's AlexaFluor488 (Catalog No. 4416S) secondary antibody to obtain remarkably excellent quantifiable images of various tissue cross-sections.
Immunofluorescence
Spleen tissue cross-section
1/100 dilution at 4 deg C in the dark overnight
Blocking buffer used was PBS with 0.05% Tween 20, 1% BSA, 5% Host serum and 0.3 M Glycine
Anti-rat Alexa Fluor 488 (Catalog No. 4416S from Cell Signaling Technologies), 1/200 for 1 hour at room temperature in the dark
Used Hoechst 33343 for nuclear staining
FITC filter in any standard epifluorescence microscope
As seen in the image, the overlay indicates positive CD68 staining after subtracting using the IgG control staining around cells (made visible via the nuclear stain - Hoechst 33342)
None
Excellent staining and positivity for macrophages
Using this combination of antibodies resulted in a fantastic set of images for various tissues in the body.