Life Technologies
SYBR® Green PCR Master Mix
4309155
Retroviruses are critical in most therapeutic and gene transduction experiments. To optimize their performance, quantifying their titer post production and purification as well as in in vivo experiments can be time consuming. Using the SYBR green PCR master mix reduced the time used to whip up multiple PCR reaction tubes by >10-fold, especially in the initial phase of protocol development wherein different concentrations of primers and starting cDNA template were to be optimized.
Retroviral Quantification
cDNA from viral RNA of viruses obtained in the supernatant of producer cell lines. See (http://www.clontech.com/US/Products/Viral_Transduction/Retroviral_Packaging/High-Titer_Retrovirus & https://www.qiagen.com/us/products/catalog/sample-technologies/rna-sample-technologies/viral-rna/qiaamp-viral-rna-mini-kit/
Simple mixing of reagents is required as per manufacturer's instructions. The master mix is supplied at 2X concentration. Optimization of primers and cDNA amount is required and is independent of this product.
Clean lab bench without genetic contamination is necessary.
Efficient (~100%, slope=(-3.29 - -3.34) amplification is obtained upon using this PCR master mix at 2-fold dilution series (top, attached image), 5-fold dilution series (middle) and 10-fold dilution series (bottom). Blue dots show the standards.
None
Easy to use. stores well
Use this product to minimize hassles related to real-time PCR protocol optimization.