Abcam
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)
ab181448
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Our work focuses on gene and protein changes (epigenetic modulators) involved in neurotrophic and neuroprotective activity. We used this antibody to check protein expression changes in neuronal cells using immunofluorescence.
Immunofluorescence
N2A cells
As per the primary antibody used
2% BSA in PBST
1:400 Rabbit alexa 488, 2h Room temperature
NA
Fluorescence microscope
Rabbit Alexa488 stained the N2a cells for protein of interest
None
Good signal
Standardisation of concentration and background noise
Works well