FGFR Antibody to Locate the Presence of FGFR1

June 30, 2015

Biology
University of Ottawa
PhD student

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Company:

Novus Biologicals

Product Name:

FGF R1 Antibody (M19B2) [Alexa Fluor ® 488]

Catalog Number:

NB600-1287AF488

Info: View Product View Product Specs

Browse Similar Products: FGF R1 Antibodies

Our laboratory selects aptamers for various cell receptors. To test the specificity of aptamer binding to white blood cells or transfected HEK-293 or MDA-MB-231, we use receptor specific antibodies. Aptamers can be labeled with fluorescent dyes or biotinylated. To detect biotinylated aptamers conjugated streptavidin is used. All cells are stained with propidium iodide or DRAQ7 to distinguish live/dead cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

300000 cells in 100 ul

Primary Incubation

1 ul per sample, DPBS buffer with Ca and Mg, room temperature, 90 min incubation

Blocking Agent

1% FBS in DPBS with Ca and Mg

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Beckman Gallios flow cytometer

Results Summary

Red - positive cells, Green - negative cells, Blue - negative cells + Ab, Pink - positive cells + Ab

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Approximately 1 order of magnitude shift compare to negative cells.

The Bad

Blocking needs to be adjusted to prevent non-specific binding

The Bottom Line

As it can be seen from the figure, there was some binding to negative cells. Probably, a different blocking agent should be used to eliminate this problem.

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