BioLegend
APC/Cy7 anti-mouse CD4 Antibody
100413
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The objective of this experiment was to study T cell subsets in mouse spleens. I tested if the anti-CD4 APC/Cy7 antibody from BioLegend can be used as an antibody to identify the CD4 positive populations. Single cell suspensions isolated from mouse splenocytes were stained with this antibody and analyzed by flow cytometry. The result demonstrates that I was able to separate CD4 positive cells from rest of the splenocytes.
Flow Cytometry
Mouse splenocytes
30 minutes
NA
BD Facs Canto II
The results show a clear CD4 positive and negative population which confirm the specificity of the antibody
None
Very good CD4 antibody
Good anti-mouse CD4 APC/cY7 antibody