Good quality DNA for cloning

Osmania University
Department of Biochemistry
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Epicentre Biotechnologies an Illumina Company

Product Name:

Masterpure DNA Purification kit for Blood Version II

Catalog Number:

MB711740

We purify DNA from mouse blood for cloning purposes. We tend to get lot of RNA contamination by conventional phenol-chloroform methods. We opted to use this kit for getting high quality of DNA from 200 microliters of blood sample.

Experimental Design and Results Summary

Application

Cloning

Starting Material

Blood

Protocol Overview

We followed the manufacturer's protocol without any modifications

Tips

Try to use fresh blood sample collected in an EDTA coated Vacutainer rather than a haparin coated one.

Results Summary

We got a very good yield of DNA from just 200 microliters of whole blood sample.

Additional Notes

None

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Summary

The Good

Very simple to obtain good quality DNA for cloning purpose

The Bad

None

The Bottom Line

I strongly recommend this product if you are going to be cloning with Genomic DNA

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