Avanti Polar Lipids Inc
18:1 Liss Rhod PE
810150
Visualization of gene therapy vectors is critical for quantitative evaluation of their functional performance in various cells. Here, we show that the lipid formulation of DOTAP, DOPE (with up to 3% of the fluorescent lipid 18:1 Liss Rhod PE) when combined with retroviral particles can be efficiently observed using fluorescence microscopy up to 4 h post transfection.
Fluorescence Microscopy, Gene Therapy
Cellular-derived viruses - See (http://www.clontech.com/US/Products/Viral_Transduction/Retroviral_Packaging/High-Titer_Retrovirus) and human cell lines (HEK293)
Simple mixing protocols were used wherein pre-determined number of retroviral particles and synthetic liposomes formulated with the fluorescent lipid were mixed and incubated for 2 h at 4 deg C. See paper for more details - http://www.ncbi.nlm.nih.gov/pubmed/25883029
Did not use 6 months post purchasing the fluorescent lipid. Use fresh and aliquot as needed.
The fluorescent lipid was visualized up to 4 h post transduction as seen in the confocal microscopy image attached. 1 - shows detached viral particle, 2 - intact gene therapy vector, 3 - shows detached synthetic lipid formulation of the fluorescent lipid
None
Easy to use. Excellent fluorescence signal intensity.
highly recommended fluorescent lipid for easy confocal microscopy of lipid formulations of drugs and gene therapeutics, especially if using phospholipids.
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