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Anti PD-L1 PE/Cy7 antibody
124314
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We are assessing consequences of Aryl hydrocarbon Receptor (AhR) activation in dendritic cells. Specifically for these experiments we were assessing Aryl hydrocarbon Receptor activation in bone marrow derived dendritic cells. Precursor cells are isolated with Lympholyte M from whole bone marrow suspensions and grown in the presence of GM-CSF for 5-7 days. We then use flow cytometry and RT-PCR to assess changes in dendritic cells after AhR activation. Here, we are assessing the expression of PD-L1 (CD274) on bone marrow derived dendritic cells.
Flow Cytometry
Murine BMDCs
1:100 at 4 degrees celsius for 25 minutes
Fc Block
NA
FACSAria II
This antibody gave us a shoulder population for PD-L1. It was difficult to get separation of positive and negative peaks; however, other cell types may give different results.
None
Works well with beads, and no compensation issues or non-specific binding
Titrations don't yield clean results
I would recommend this product.