Novus Biologicals
Frizzled-7 Antibody (151143) [Allophycocyanin]
FAB1981A
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Our laboratory selects aptamers for various cell receptors. To test the specificity of aptamer binding to white blood cells or transfected HEK-293 or MDA-MB-231, we use receptor specific antibodies. Aptamers can be labeled with fluorescent dyes or biotinylated. To detect biotinylated aptamers conjugated streptavidin is used. All cells are stained with propidium iodide or DRAQ7 to distinguish live from dead cells. HEK 293 cells were transfected with FZD7 and later used for aptamer selection. Abs were used to verify the transfection of HEK 293 cells.
Flow Cytometry
300000 cells in 100 ul
90 minutes, 5 ul per sample (100 ul), room temperature, DPBS with Ca and MG
1% FBS in DPBS with Ca and Mg
NA
Beckman Gallios flow cytometer
Transfected cells gave statistically higher signal than non-transfected cells.
None
Abs gave good signal
Blocking needs to be adjusted to prevent non-specific binding
In our experiment Abs worked well to verify the transfection.