To measure the HDAC activity/Inhibition

indian institute of chemical technology
chemical biology

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Epigentek

Product Name:

Epigenase HDAC Activity/Inhibition Direct Assay Kit (Colorimetric)

Catalog Number:

p-4034-48

We do work on gene and protein expression changes (epigenetic modulators) in neuroglia differentiation and behaviour. This product was used to measure HDAC activity/inhibition in samples treated with HDAC inhibitor.

Experimental Design and Results Summary

Application

HDAC activity/inhibition

Starting Material

Gl-261

Protocol Overview

Protocol followed as per the user guide provided by the company

Tips

Store the reagents as suggested in user guide, reagents prepared freshly while using take more protein(5-10ug), after adding the SS solution take reading as soon as possible

Results Summary

In HDAC inhibitor treated samples the HDAC activity is less compared to the untreated control.

Additional Notes

Make the purified enzyme or nuclear extract ready before using the kit, make the required dilutions of HDAC inhibitors

Related Categories

Image Gallery

Summary

The Good

Easy to handle

The Bad

Cost, HDAC enzyme or nuclear extract is not supplied to screen the HDACis

The Bottom Line

Can be used to asses the HDAC activity/inhibition.

Join the discussion