R&D Systems
Human TNF-RII ELISA kit
DRT200
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I am interested in whether yogurt consumption could decrease chronic inflammation in obese populations. Previous reports have shown a decreased ration of TNF-alpha over sTNF-RII to be an indicator of suppressed inflammation. Therefore, I measured both TNF-alpha (a kit also from R&D, high sensitive version) and sTNF-RII in my samples.
For sTNF-RII measurement in human plasma
Human plasma
1. Prepare wash buffer, sTNF-RII standard. 2. Add assay diluent. 3. Add standard and diluted samples (I used 11 times dilution for my plasma sample from a healthy population). 4. Incubate. 5. Wash the plate. 6. Add conjugate and incubate. 7. Repeat wash procedure. 8. Add substrate which should be prepared within 15 mins of use and keep from light. 9. Add stop solution. 10. Read.
1. Prepare standard in polypropylene tubes. 2. Remember to mix the color reagent (substrate solution) within 15 mins of use.
The kit is pretty stable. I have inter-plate CV of around 5%.
Remember to dilute your samples with the right diluent.
Instructions are straightforward.
Not much to complain about.
I will buy this kit again.
Product Name: Human sTNF RII/TNFRSF1B Quantikine ELISA KitSupplier Page