S1P1 Abs for white blood cells

Our laboratory selects aptamers for various cell receptors. To test the specificity of aptamer binding to white blood cells or transfected HEK-293 or MDA-MB-231 we use receptor specific antibodies. Aptamers can be labeled with fluorescent dyes or biotinylated. To detect biotinylated aptamers conjugated streptavidin is used. Here, white blood cells were stained with propidium iodide and S1P1 Abs to perform screening of anti-S1P1 aptamers.