Western blot using Anti-Human/Mouse Foxp3

May 27, 2015

Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

eBioscience

Product Name:

Anti-Human/Mouse Foxp3 Purified

Catalog Number:

14-5773-80

Info: View Product View Product Specs

Browse Similar Products: FoxP3 Antibodies

I used this antibody to analyze FoxP3 protein expression in mice CD4+ T cells under different stimuli.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse CD4+ T cells

Primary Incubation

1:500, Overnight at 4C

Blocking Agent

5% Milk

Secondary Incubation

Anti-rat antibody (1:5000) for 1 hour at room temperature

Tertiary Incubation

NA

Detection

ECL

Results Summary

I got nice Western blot results without any non-specif bands and background.

Additional Notes

None

Related Categories

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Summary

The Good

Easy optimization and no non-specific bands

The Bad

Requires higher concentration.

The Bottom Line

I got pictures of publication quality with this antibody. Good and worth buying.

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